The effect of troxerutin on lipid peroxidation and tissue injury induced by myocardial ischemia reperfusion injury in diabetic rat

Diabetes is a main risk factor of ischemic heart disease. Troxerutin, a natural bioflavonoid rutin, has many biologic properties, such anti-oxidative and anti-inflammatory effects. The purpose of this study was to investigate the interaction of diabetes with the protective effect of troxerutin on lipid peroxidation and tissue injury induced by myocardial ischemia reperfusion injury in rats. Male Wistar rats (250-300 g) were divided into 4 groups: control, control+ drug, diabetic and diabetic+drug. Diabetes was induced by single injection of streptozotocin (50 mg/kg; intraperitoneally) and the diabetic period was 10 weeks. After 6 weeks of diabetes, treatment groups were received 150 mg/kg/day troxerutin in distilled water by oral gavage for 4 weeks. The hearts were removed quickly, mounted on Langendorff apparatus, and then subjected to 30-minutes regional ischemia followed by 60-minutes reperfusion. The levels of lipd peroxidation (MDA) of left ventricular supernatant and level of creatine kinase (CK) release in coronary flow were measured by spectrophotometric method using special kits. STZ increased blood glucose in the diabetic group than in the control group. Troxerutin could reduce blood glucose in diabetic group, but this reduction was not statistically significant. MDA levels were increased in diabetic rats compared to control ones and troxerutin treatment could reduce MDA levels in diabetic group (p<0.05). Diabetes causes release of large amounts of CK compared to the control group. TXR administration reduces the level of this enzyme in the diabetic group. Troxerutin reduced lipid peroxidation and tissue injury probably by preventing oxidative stress and activation antioxidative system especially in diabetic rats and can have the protective effects on damage induced by myocardial ischemia and reperfusion