Evaluation of the effect of antimicrobial activity of ethanol extract of Myrtus communis, Zataria multiflora Boiss and Allium sativum on biofilm formation by Staphylococcus aureus

Background and Development of antibiotic resistance among pathogenic bacteria motivates attempts to search for newer antimicrobial agents. In this research study, antibacterial effect of plant extract of M. communis, z.multiflora and Allium sativum on biofilm formation by strains of Staphylococcus aureus resistant to the antibiotic were investigated. Methods and Materials: 17 samples were separated from nose and throat of hospital and non-hospital personnels. The effect of extracts on biofilm formation by S.aureus and its resistant respect to the antibiotic were determined using a microplate96. Effect of antibacterial extracts used at different concentrations showed that, despite the relative resistance of most strains at concentrations used, the maximum sensitivity was at concentrations of 1.25, 2.5 and 5 mg/ml of extracts and concentration of 10 mg/ml of the extract was considered as MBC. Bacterial growth was observed only at 24 hours and no biofilm was observed at any of the extracts. Results showed that plant extracts can reduce biofilm formation by Staphylococcus aureus and, therefore, they can play an important role in reducing disease.