Design a biosensor to measure glucose by using modified electrode with cadmium oxide nanoparticles and glucose oxidase enzyme

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Abstract:
In this study، we introduced a new biosensor for measurement of glucose by using of glucose oxidase enzyme، cadmium oxide nanoparticles and carbon paste electrode. Size of cadmium oxide nanoparticles were about 43 nm. The results indicated that produced carbon paste electrode modified with cadmium oxide nanoparticles، can facilitates electron transfer in stabilized glucose oxidase enzyme on the electrode surface. In addition، the reduced form of glucose oxidase enzyme could oxidize by solutions oxygen with an electro catalytic reaction، that this oxidation، due to reaction between the oxidized form of glucose and glucose oxidase and this reaction inhibited by glucose. Based on electro catalytic decrease of glucose oxidase enzyme in saturated oxygen solution in the presence of glucose، a new sensor was designed for glucose. Designed sensor has high sensitivity and in linear range of 20 µM to 360 µM and can be used to determine glucose. This biosensor also has very good stability.
Language:
Persian
Published:
Journal of Molecular and Cellular Research, Volume:27 Issue: 3, 2014
Pages:
354 to 366
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