Quantitative Study of the Genes Involved in Survival and Autophagy Pathways in T-47D Cell Line with Emphasis on Upgrading Cold Resistance of the Cells in the Presence of DMSO

Dimethylsulfoxide (DMSO) is one of the cryoprotectants utilizing in freezing and long term maintenance of the cells. In this study، the mechanism of protection of this mater in relation with cell death and autophagy is envisaged. Study of the expression level of autophagic genes (atg5، lc3، beclin1، dram و p53)، using real time RT-PCR، indicated that at the lowest concentration of DMSO the genes involved in autophagy are over-expressed، thus regarding to death autophagy induction، the cells have lower viability relative to those cells which are freezed at the higher concentrations of cryoprotectant. Increasing cell viability، at the greater concentrations of DMSO (over than 10%)، occurs because of the converting of death autophagy to cytoprotective one which is supported by means of over-expression of the genes involved in the survival pathway. Simultaneous study of autophagy and AKT/mTOR pathway can be utilized as a separating method for death and survival autophagy.