Comparison of anti-angiogenic effect of Licorice root aqueous extract and Glycyrrhizic acid chick Chorioallantoic membrane

Angiogenesis, is an important process in physiological and pathological conditions as tumor growth and metastasis. Therefore, combinations that lead to inhibit angiogenesis may develop as an anti-cancer drug. In the present research, the anti-angiogenesis effect of Licorice root extract and Glycyrrhizic acid in Chorioallantoic membrane is investigated. In this experimental study, 45 ROSS fertilized eggs were randomly divided into three groups, Control, experimental 1 and 2. On the eighth day, Chorioallantoic membrane was soaked in control group with DMSO, in the first experimental group with extract of licorice and in the secondth, with Glycyrrhizic acid. Twelfth day photographed from samples whit using of photosteriomicroscope, the number, and length of vessels measured, also Drabkin test was performed to estimate the vessel density. Obtained data were analyzed by t-test (P<0.05). The mean number and length of vessels in samples treated with extract was showed a significant decrease compared to the control (P <0.001). The mean number and length of vessels in samples treated with Glycyrrhizic acid was showed a significant reduction compared to the control (P <0.05). Compare this parameters, in samples treated with Licorice extract to samples treated with Glycyrrhizic acid was showed a significant decrease (P <0.05). Both of, licorice extract and Glycyrrhizic acid have inhibitory effect on angiogenesis in Chick chorioallantoic membrane, however, The inhibitory effect of extract is stronger than Glycyrrhizic acid. Therefore licorice and Glycyrrhizic acid can be as suitable condidate on angiogenesis study.