Presence of alginate among multidrug resistant Pseudomonas aeruginosa isolated from clinical samples in Qazvin and Tehran hospitals

Pseudomonas aeruginosa is an opportunistic infection that can occur in burn and immunocompromised patients causing several clinical problems for specialists. Most of infection caused by this organism are associated with production of alginate exopolysaccharide and biofilm formation in targets which can increase drug resistance. The aim of this study was to evaluate the frequency of alginate among multidrug resistant P. aeruginosa isolated from Qazvin and Tehran hospitals. In total, 115 clinical P. aeruginosa isolates were collected from educational hospitals of Qazvin and Tehran hospitals during 2012 to 2014. Antimicrobial susceptibility was further determined by disk agar diffusion method. PCR and sequencing assays were performed for detection of algD gene among MDR isolates. Results of this study showed 94(81.7%) of MDR isolates were positive for the presence of algD gene. The positive isolates were mostly obtained from blood samples (28.7%) and patients admitted in ICUs (36.5%). These findings showed the high prevalence of algD among MDR P. aeruginosa isolated from admitted patients in studied hospitals. According to clinical importance of these isolates, using an appropriate infection control policy and therapeutic strategy is necessary.