Effects of extender and sperm chamber on computer-assisted analysis of sperm motility (CASA) in goats

Computer-assisted semen analysis (CASA) is used as an accurate and objective tool for kinetic sperm measurements. The aims of this study were evaluate the effects of extender and sperm chamber on motility parameters of goat sperm using CASA. Semen collected from five Mahabadi goats via artificial vagina. The collected semen divided in two fractions and diluted in two commercially soyabean based extender، Bioxcell and Andromed to finally concentration of 6 × 106 spermatozoa per ml. The diluted semen evaluated via CASA and using two sperm chambers including Makler chamber with 10µm deep and Leja chamber with 20µm deep، and microscopic slide with 18*18mm cover. The motility parameters considered in this study were average path velocity (VAP، µm/s)، straight line velocity (VSL، µm/s)، curvilinear velocity (VCL، µm/s)، amplitude of lateral head displacement (ALH، µm)، beat cross frequency (BCF، Hz)، straightness (STR، as VSL/VAP، %)، and linearity (LIN، as VSL/VCL، %). The medium used for the dilution of the sample significantly affects all sperm motility parameters except VCL. Only ALH was significantly lower (P < 0. 05)، whereas VSL، VAP، BCF LIN and STR were significantly higher، in the semen diluted in Bioxcell than Andromed. The effects of sperm chamber were significant only in LIN and BCF. In conclusion the extender had significant effect on the CASA results whereas sperm chamber didn''t has so effects.