Ruminal disappearance of protein and amino acids of untreated and treated canola meal with gamma ray and electron beam

This study was conducted to investigate the effects of doses of 25، 50 and 75 kGy of gamma ray (GR) and electron beam (EB) ionizing radiations on ruminal disappearance of amino acids (AAs) and protein subunits of canola meal (CM). The nylon bag technique was used for degradability trial. Three ruminally fistulated Taleshi bulls were used for this aim. The disappearance trends of protein subunits for protein meal samples were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Irradiation decreased ruminal degradability of AAs (P<0. 05). In this case، except for serine، tyrosine and glutamate، the effect of GR in reducing AAs degradability was more than the EB. Electrophoresis analysis identified the presence of cruciferin with four subunits in CM. In unirradiated CM، all four protein subunits of cruciferin were degraded after 8 h of rumen incubation. Doses of 25، 50 and 75 kGy of EB and GR preserved the protein subunits of cruciferin up to 16، 24 and 48 h، respectively. It can be concluded that processing with ionizing radiation of EB and GR decreases ruminal degradation of CM protein subunits of cruciferin and AAs. The effect of GR in reducing ruminal degradation of CM proteins is more than EB.