Effects of growth regulators on in vitro regeneration of Ziziphus jujube

Ziziphus jujuba belongs to Rhamnaceae and consist 50 genus and 600 species that grow in European and tropical areas. Fruits of the species possess medicinal potentials. For propagation of Z. jujuba micropropagation was investigated. Various sterilization techniques، culture conditions، and different hormonal treatments on pollution، regeneration، proliferation and root induction were studied. Apical and axillary buds were used as explants. Buds were sterilized by 0. 02% mercuric chloride for 3 min، 70% ethanol for 2 min and 30% NaOCl for 15 min. To evaluate the culture conditions، the explants were cultured on MS medium supplemented with different hormones such as IAA، BA، IBA and TDZ. Results indicated that MS basal medium supplemented with 0. 01 mg/L IBA + 2 mg/L BAP was suitable to obtain shoots from axillary buds (96. 9%). Apart from that، there was no significant differences (p>0. 05) among the studied media for multiplication. Root induction from shoots was observed at concentration of 5 mg/L of IBA in MS medium.