In vitro plant regeneration via somatic embryogenesis of Securigera securidaca

Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from cotyledon، hypocotyl and root explants excised from in vitro seedlings of Securigera securidaca. The explants were cultured in MS medium supplemented with various concentrations of BAP or TDZ in combination with various concentrations of NAA in a factorial experiments based on a completely randomized design with four replicates. Twelve explants per replicate were studied. No callus was produced on control (hormone-free medium) treatment، while well developed calli with proembryogenic structures were obtained using BAP or TDZ in both cotyledon and hypocotyl explants. Although، TDZ in combination with NAA was the most effective on somatic embryo regeneration in calli derived from all studied explants، however، it was more efficient on callus production in root explants and subsequently embryo regeneration. The protocol described in the present study can be used for the production of a large number of true-to-type plants and production of secondary metabolites of pharmaceutical values of S. securidaca.