Protective Effect of Nanoceria against Streptozotocin Induced Mitochondrial Dysfunction in Embryo of Diabetic Mice

Background and Gestational diabetes is known as increasing blood glucose level for the first time during pregnancy. Mitochondrial damage and oxidative stress are the most important factors in the development of diabetic complications. Cerium nanoparticles have antioxidant properties. In this study we examined the protective effect of nanoceria in preventing mitochondrial damage induced by diabetes. In this study, the mice were divided into five groups including control, diabetic, Nanoceria, diabetes+Nanoceria, and diabetes + vit E groups. Diabetes was induced by STZ (60 mg/kg IP). Blood glucose level was checked in days 1,5,10, and 15 of pregnancy. The diabetic state was confirmed when the blood glucose concentration exceeded 200 mg/dl. On day 16 of pregnancy the embryos were excised and their mitochondria were isolated using different centrifuge technique. Then, oxidative stress markers and mitochondrial damage were measured. Diabetes significantly increased the production of reactive oxygen species, lipid peroxidation and oxidation of glutathione in isolated mitochondrial in embryo of diabetic mothers (P<0.05). Nanoceria treatment (60 mg/kg) significantly prevented the mitochondrial oxidative stress induced by gestational diabetes in embryo of mice (P<0.05). Also, mitochondrial function significantly decreased in diabetic group compared to that of the control group, which was caused by nanoceria treatment that significantly inhibited diabetes-induce mitochondrial toxicity (P<0.05). Cerium oxide nanoparticles had significant effects in preventing oxidative stress and mitochondrial damage induced by gestational diabetes and could be considered in reducing the effects of gestational diabetes.