Study of the efficacy pegylated Cisplatin loaded nano archaeosome on breast cancer in vitro

Aim and Archaeosome nanoparticles are used in drug delivery to improve the efficacy of therapeutic agents and decrease the side effects. In this study, archaeosome nanoparticles were used as Cisplatin carrier. Methanogenic archaea were successfully cultured in a routine liquid medium. Archaeosome was extracted from the membrane of archaea using centrifugation process. After that homogenous emulsion of archaeosome was raised in phosphate buffer saline containing poly ethylene glycol and Cisplatin under motion by stirrer. Sonication performance was also recruited to obtain the nano sized archaeosome and Cisplatin entrapment. While Cisplatin encapsulation efficiency was estimated in supernatant after separation by ultracentrifuge, cytotoxicity effects of Cisplatin in the free form and encapsulated on the archaeosome were calculated in the breast cancer MCF-7 cell line using MTT assay. Drug-containing nanoparticles were synthesized successfully. The entrapment efficiency for pegylated and non pegylated nano archeasomes was estimated 70.16 and 54.52% respectively. Size of the nanoparticles was shown to be 132/84 and 388.31 nm for non pegylated and pegylated nanoparticles respectively. Although cytotoxicity effects of Cisplatin was increased when drug become associated with NPs, this effect was predominant when pegylation process was contributed to construct the Cisplatin loaded nano archaeosome. The results of our study suggested that archaeosome nanocarriers could be used as a suitable carrier for Cisplatin while pegylation increases the efficiency of preparation.