Evaluation of antioxidant and cytotoxic activity of local actinomycetes Print

Aim and Among more than 23,000 microbial secondary metabolites discovered, 42% of them are separated from actinobacteria. These secondary metabolites have wide range of biological activities such as antibacterial, antifungal, antiparasitic, antiviral, antitumor, immunosuppressive, insecticide, antiinflammatory, antioxidant and other activities. In the past few years, numerous studies have been conducted on these biological activities in the country, but no report has been published about the antioxidant activity of local actinomycetes resources. The purpose of this research is to achieve natural antioxidants with lower physiological hazards associated with local microbial resources. Fifty strains of native actinomycetes preserved at the University of Tehran Microorganism collection were selected randomly and after sporolation and prefermentation and fermentation, bacterial extracts were prepared. At first, antioxidant activity of the extracts was evaluated by DPPH method. These results were evaluated with synthetic antioxidants including ascorbic acid and BHA. Also cytotoxic activity of the extracts was measured on Artemia. Elected strains were recognized with molocular method. According to the results, 10% of extracts have no antioxidant activity, 20% of the extracts have high antioxidant activity (IC50<200), 32% of extracts with medium antioxidant activity (200