The Effect of Exercise with and without Consumption of Hydroalcoholic Extract of Eriobotrya Japonica Flower on Mesencephalic Astrocyte-derived Neurotrophic Factor in the Cerebellum of Rats Subjected to 6-Hydroxydopamine

Previous researchs has shown that exercise and antioxidants consumption have positive effect on oxidative stress. Therefore, this study was carried out aiming at investigating the effects of 12 weeks of exercise on a running wheel, with and without consumption of the extratct of Eriobotrya japonica flowers, on MANF level in the cerebellum of rats subjected to 6-hydroxydopamine (6-OHDA). Rats were divided into 6 groups: Base, control-treatment, training-healthy, training-treatment, extract-treatment, and training-extract-treatment. Training groups were housed individually in cages with attached running wheels, and the extracts group received 200 mg/kg bw of the extract, 3 times per week during the research period. The 6-hydroxydopamine solution (250 mg/kg bw) was stereotaxically injected into intracerebroventricular (ICV) region of the brain. MANF levels in the cerebellum were measured by ELISA. Data were analyzed using one-way ANOVA and post-hoc LSD test. In this investigation, the voluntary training on running wheel along with consumption of the extract significantly prevents the decrease in MANF levels in pre-treatment groups, (p=0.031) and (p=0.022). The difference between the control-training group and supplementation group as well as other four groups was statistically significant (all p<0.05). The results of the present study showed that pre-treatment with voluntary training or extract of Eriobotrya japonica flower increases neuroprotection against the stress induced by ICV injection of 6-OHDA.