Effects of the BAP, Kin and GA3 on the in vitro shoot regeneration and effects of the activated charcoal and IAA on in vitro rooting in lisianthus plant (Eustoma grandiflurom)

Lisiantus is introduced among the 10 top cutting flowers in the world. Micropropagation can be used in this plant because of some problems in traditional propagation. In this research، the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. The effects of the different concentrations of BAP and Kin on shoot regeneration in MS medium were investigated according to a completely randomize design (CRD) in the independent experiments. The results indicated that the use of 0. 5 mg l-1 BAP was the best treatment for shoot regeneration (4 shoots per explant). In the next experiment، the interaction of BAP and GA3 on shoot proliferation were investigated according to a factorial experiment based on CRD. The results showed that the best shoots in terms of quality and quantity (17 shoots per explant) were obtained in MS medium supplemented with 0. 5 mg l-1 BAP and 0. 2 mg l-1 GA3. The shoots produced in this treatment were transferred to MS medium containing different concentrations of IAA (0، 0. 2، 0. 5 and 1 mg l-1) and activated charcoal (0 and 3 g l-1) to investigate the shoot rooting. MS medium supplemented with 1 mg l-1 IAA and without activated charcoal was determined as the optimum medium for shoot rooting.