Production of pyocin by Pseudomonas aeruginosa and investigation of its synergism effect with some common antibiotics in vitro

Aim and Pseudomonas aeruginosa is an important cause of nosocomial infection. According to widespread antibiotic resistance and limitation of effective drugs, nowadays much attention has been focused on the use of alternative drugs. One of the alternatives is pyocin of Pseudomonas that can have inhibitory effect on relative species and even on other genus. This study was aimed to study the production and investigation of antimicrobial effects of pyocin on some pathogenic bacteria and investigation of its synergism effect with some common antibiotics. Production of pyocin by isolated strains of Pseudomonas aeruginosa was investigated without stimulator and in presence of mitomycin C in various temperature and pH values. Inhibitory effect of pyocin was studied on some pathogenic bacteria; also synergism effect of pyocin with some antibiotics was investigated. Pyocin production was done both in the absence of stimulator and to a greater extent in the presence of mitomycin C. Optimum temperature for the production of pyocin was 37C and optimum pH was 7. Produced pyocin showed a significant inhibitory effect on target bacteria and had synergism effect with some studied antibiotics. Pyocin can have an efficient inhibitory effect on pathogenic bacteria in vitro. Upon further investigations especially in vivo, more detailed information can be obtained about the possibility of its using as an alternative or complement for the treatment of bacterial infections.