Frequency of fliC, algD genes from clinical Pseudomonas aeruginosa strains, isolated in Zabol

Pseudomonas aeruginosa is an opportunistic bacterial pathogen and one of themost frequent bacterial agents in nosocomial infections. It causes different types of infections including urinary tract infections and bacteremia. This bacterium has manypathogenic factors including; exotoxin A، lipopolysaccharide، phospholipase C، pili،elastase، flagelline، alginate، and alkaline protease. In this study،frequency of fliC and algD genesin clinical Pseudomonas aeruginosa strainsisolated from nosocomial infections were analyzed. 100 Pseudomonas aeroginosa strains were isolated in clinical samples of Amiralmomenin Imam Ali hospital in Zabol. The isolates were identified by biochemical methods، afterwards DNAs were extracted. In order to detect presence of fliC and algD genes among the isolated bacterial strains، special primers were designed and PCR was performed. Results showed 80% strains have fliC and 95% algD، respectively. The results of our study confirm that fliC and algD genes as important virulence factor of Pseudomonas aeruginosa، present in isolated bacterial strains considerably. The results of our study showed that alginate gene is so important in pseudomonas aeroginosa strains isolated from urinary tract infectionsand burnwound infections and it can be used for detection of bacteria and also can be used as a vaccine target against this bacterial pathogen.