Effect of bovine serum albumin on Caspian horse sperm quality during cooling

The aim of this study was to evaluate the effect of bovine serum albomin (BSA) in stallion semen extender in cool condition. After collection, semen samples were pooled and divided into four equal parts. Each part was diluted with one of the following extenders: 1) extender without BSA (BSA-0), 2) extender containing 5 mg/mL BSA (BSA-5), 3) extender containing 10 mg/mL BSA (BSA-10), and 4) extender containing 15 mg/mL BSA (BSA-15). After 2, 24 and 48 hours in cool condtions, the effect of different extenders on motility, viability, plasma membrane functionality and Malondialdehyde (MDA) level were evaluated. The obtained results indicated that total sperm motility in BSA-5 and BSA-10 extenders was significantly higher compared to BSA-0 and BSA-15 extenders after 48 hours. BSA-10 extender resulted in significantly higher progressive motility compared to BSA-0 and BSA-15 extenders. Plasma membrane functionality was significantly lower in BAS-15 extender compared to other extenders after 48 hours. Viability was significantly decreased in BSA-15 extender compared to other extenders. In conclusion, BSA-15 extender had lower protective effect on total motility and plasma membrane functionality comared to other extenders at 48 hours.