Evaluation of qualitative and quantitative of antocyanines, carotenoids, flavonoids and antioxidant activity of methanol extract from aerial parts of Scutellaria pinnatifida A.Hamilt subsp alpina (Bornm) Rech.f

Scutellaria pinnatifida from Labiatae family is one of the endemic medicinal plants in Iran and is used as anti analgesic and anti-inflammatory remedy. The aim of this study was evaluation of antioxidant activity and phytochemical assessment of Scutellaria pinnatifida A.Hamilt subsp alpina (Bornm) Rech.f that is endemic in Khorasan. the methanol extract from aerial parts of plant was provided with maceration method at first. The presence and amount of Flavonoids, Tannins, Saponins, Antocyanins and Carotenoids was studied. Antioxidant activity of extract was evaluated with Free radical scavenging and Ferric reducing antioxidant power methods. the result of this study showed the presence of antocyanin and flavonoids and absence of saponin and tannins. The amounts of antocyanin and flavonoid were 7.65 ±0.13 and 10.86 ± 0.0115 mg/per g dry plant respectively.Free radical scavenging was increased with high concentration of extracts and highest scavenging was in 32 mg/ml with 85.4% scavenging, BHT and Vit C were 91.58% and 95.33% in this concentration and there wasn’t more different between them. In evaluation of antioxidant activity in FRAP method, methanol extract with 0.816±0.052 mmol ferrous was stronger than BHT and it is weaker than Vit C. the methanol extract from aerial parts of Scutellaria pinnatifida had good antioxidant activity and more studies are needed for recognize of it's chemical compositions and evaluation of anticancer activity from this plant.