Antimicrobial Activity of Rosmarinus Officinalis on Vancomycin -Resistant Staphylococcus Aureus Isolated from Imam Reza Hospital Patients of Mashhad

Introduction and Rosemary (Rosmarinus officinalis) belongs to Lumiaceae family and contains different properties and compounds. With emergence of Vancomycin resistance Staphylococcus aureus, it has been confirmed as a global issue. The aim of this study is the survey of methanolic extract of rosemary leaves on Vancomycin -resistant Staphylococcus aureus isolated from patients of Imam Reza hospital of Mashhad. 150 bacterial samples have collected from Imam Reza hospital of Mashhad at the beginning of 2013 November. One of them was being resistance to Vancomycin antibiotic by Disk Diffusion and MIC (Minimum Inhibitory Concentration). Extracting from Rosemary was performed by Maceration method. The MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal concentration) has evaluated by reference method of Microdilution Broth. The result of this study demonstrated that the MIC and MBC for Methanolic extract of Rosemary on Vancomycin -resistant Staphylococcus aureus were 0.156×103 and 0.312×103 mg/ml respectively. Bacterial resisting to antibiotics implies necessity of using of new compounds with antibacterial property. The methanolic extract of rosemary can inhibit the growth of Vancomycin resistance Staphylococcus aureus. Therefore it can be used as clinical purposes.