Isolation of Honey Bee's Pathogenic fungal Agents in Guilan Province

This study was conducted to isolate honey bee's pathogenic fungal agents in three seasons (spring, summer and autumn) in Guilan province apiaries. Randomly, we selected five regions and then ten apiaries from each region and four colonies of each apiaries sampled from adult honey bees, brood, honey and pollen and transported to the laboratory. All samples cultivated in SDA (Sabouraud Dexterose Agar) medium. Out of 1469 samples, 76 samples (5.17%) were positive to Honey bee disease fungal agents, 2 (0.14%) Ascosphaera apis causative agent of chalk brood disease and 74 (5%) Aspergillus spp. the causative agent of stone brood disease was isolated. Contamination percent rate in pollen (2.38%), adult honeybees (2.11%), brood (0.48%) and honey)0.2%) were reported. Contamination percent rate to the Honey bee pathogenic fungal agents in summer was reported more than the other seasons.