The effect of adding essence and leaf of two species of Oregano on gas production parameters, digestibility, and ruminal fermentation parameters in in vitro

A study was conducted to evaluate the effect of adding essence and leaves of two species of Oregano (Mentaaquatica and Mentalongifolia) on gas production parameters, digestibility of dry matter and organic matter and microbial biomass production in two experiments separately. Collected two species of Oregano was taken essence by macro wave set at 110 0C and 450 nm for 45 minutes. The experimental treatments were: 1) control containing basal diet with forage: concentrate ratio 50:50 (without additive), 2) basal diet + Mentaaquatica lamiaceae essence, 3) basal diet+ Mentalongifolia lamiaceae essence, 4) basal diet+ Mentaaquatica lamiaceae leave and 5) basal diet+ Mentalongifolia lamiaceae leave. 8 macro liter of essence along 8 macro liter of ethanol in treatment 2 and 3 and 20 mg leaves of two kind of Oregano in treatment 4 and 5 were added to glass vial. The results showed that treatments containing essence and leaves had lower gas production than control treatment. Hence, treatment control and treatmenl) gas production, respectively. In second experiment, there were significantly differences aents that containing leaves of two kind of Oregano had lowest DM and OM digestibility compared with other treatments. Treatments containing essence had higher microbial biomass production and microbial biomass production efficiency. Generally, it can be concluded that essence of two kind of Oregano (even their leaves) has the potential to affect ruminal fermentation efficiency.