Consideration of protective effects of Butylated Hydroxytoluene (BHT) in semen extender on the fertility and quality parameters of frozen-thawed Rooster semen.

Study of the effect of adding different concentrations of butylated hydroxytoluene (0, 0.5, 1, 2 and 4 mM) on the cryopreservation of rooster sperm in the diluent based on soya lecithin. In this research, semen samples from five mature rooster were collected by using the method of abdominal massage and after collection they were diluent based on soya lecithin and were frozen in 0.25 ml straw. After the freezing process, the parameters such as total motility, progressive motility, sperm viability, sperm morphology, sperm membrane integrity and the amount of producing malondialdehyde were investigated after freezing-thawing process. Results of these experiments indicate that the diluent containing 1, 2 mM of butylated hydroxytoluene produced high percentage of mobility, progressive mobility, survival, and membrane integrity and normal sperm morphology in comparison to the rest of other concentration. Although there was no significant difference in the amount of malondialdehyde, in this experiment, we came to the conclusion that the levels of 1 and 2 mM of butylated hydroxytoluene have the appropriate protective effects for sperm rooster.