Effect of hydrogen peroxide on induction of secondary metabolites in Periwinkle (Catharanthus roseus L) callus

Aim: In the present study the effect of oxidative stress resulted from hydrogen peroxide on some secondary metabolites and changes on intra cellular hydrogen peroxide has been explored.
Material and To induction of callus Periwinkle leaf was sterilized and put on MS medium. Calluses were treated by different concentrations (1, 5 and 10 µM) of hydrogen peroxide for 6 days. Total alkaloid, phenolic components and felavonoids as well as antioxidant potential on the basis of Iron reduction were determined. Data were analyzed using one way ANOVA.
Addition of hydrogen peroxide resulted in significant (P≤0.05) increase of intra cellular hydrogen peroxide compared to the control. Total alkaloids, flavonoids and phenols content showed that hydrogen peroxide caused a significant increase (P Hydrogen peroxide increased intra cellular levels of hydrogen peroxide. Secondary metabolites such as alkaloids and phenolic compounds and total flavonoid were boosted as well. Therefore tensions may be applied to increase them.