Synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine - Dendrimer in Wilson's model cells

Wilson’s disease (WD) is caused by mutation to the cooer-transporting gene ATP7B. Chelation therapy is the main protochol of treatment for patients with Wilson’s disease. D-penicillamine is one of the well-known chelator agants which is used in WD treatment but it can not enter into the intracellular space.This study was done to evaluate the synthesis and anti-intracellular Copper overload evaluation of Nanoconjugated D-penicillamine –Dendrimer in Wilson’s model cells.
In this descriptive-analytic study¡ initially 0.01 mm polyethylene glycol (PEG) and 0.0018 mm citric acid¡ Dendrimer was synthesized. After purification by dialysis bag and lyophilization¡ 10mg dendrimer was conjugated to 3.3mg D-penicillamine. Nanoconjugated D-penicillamine-dendrimer was injected on Wilson’s model cells. After incubation and centrifugation intracellular measurement of copper concentration and FTIR test were done.
Copper accumulation significantly reduced in the HepG2 WD cell by Nanoconjugated D-penicillamine - Dendrimer in compared to D-penicillamine (P Nanoconjugated D-penicillamine –Dendrimer can reduces intracellular concentration of Copper.