Long-term effects of green tea extract on the damage caused by Doxorubicin in rat bone marrow

One of the most well known anti-cancer drug is Doxorubicin. However, green tea has many antioxidant properties. In this study, the long-term effects of green tea on bone marrow lesions caused by Doxorubicin in rats were studied. 30 adult male Wistar rats were randomly divided into 6 groups. Control, LGT (long-term use of 3% green tea extract for 60 days), SHGT (shortterm use of 3% green tea extract for 10 days), LGT DXR (long-term use of 3% green tea extract for 60 days with injections IP doxorubicin for 3 days), SHGT DXR (short-term use of 3% green tea extract for 10 days in combination with doxorubicin (IP) injection for 3 days), DXR (doxorubicin injection for 3 days). 24 hours after the last injection of DXR, rats euthanized and tissue samples were taken for histopathology of the femur. The enzymes catalase and superoxide dismutase (SOD) were measured. The histopathology results show that the DXR induced severe bone marrow suppression that myeloid cells was more involved. SHGT DXR group was similar to DXR, but the cell density was similar to control group. The LGT DXR cell density was very similar to the control group. There was a small amount of myeloid cell destruction. Measurement of catalase and SOD indicates a significant difference between treatment and control groups. Results show that Doxorubicin may cause damage in rat bone marrow, but long-term use of green tea can reduce the damage caused by doxorubicin.