Molecular characterization of F gene and protein of Newcastle disease viruses isolated in 2008 and 2009 in Iran

Newcastle disease is one of the most important diseases in poultry with huge economical damages. It is possible to detect virulence of virus base on molecular techniques and sequence of F gene. In this study, Sequence of 1674 nucleotide acid in cleavage site of F gene of Newcastle disease virus¡ was analyzed for 10 isolates in industrial poultry. These isolates were obtained from endemic of very virulent Newcastle disease in Iran during 2009 and 2010 which have caused high mortality in broiler, breeder and layers along with high egg production drop in breeders and layers. Amino acid sequences were compared phylogenetically with those of previously reported in Iran and also with the gene bank. These isolates show phylogenitically distinction from previous isolates reported in Iran. These isolates show similarity with Chinese and Israeli isolates in gene bank.