The comparison of ELISA and PCR for laboratory diagnostic of bovine leukosis

Bovine leukemia virus (BLV) from deltaretrovirus genus and retrovirus family has three structural genesnamed gag, pol, env. 200 whole blood and serum samples from affected cattle over two years from different regions of Iran were examined for BLV provirus in two methods. At the first method, samples were detected through indirect ELISA and 74 cases (37%) had antibody against gp51 antigen. The second method, 30 positive serum samples and 20 apparently healthy cattle (negative serum in ELISA) were examined by PCR method for provirus of BLV. In this study, all of positive serum and 3 negative serum samples showed specific 427 bp segment of BLV provirus.