Evaluation of Endogenous Nitric Oxide Effect on Water Intake in Cholestatic Rats: An experimental study

Cholestasis stops the flow of bile from the liver to the deudenum. Cholestasis is associated with the over production of endogenous nitric oxide (NO), and water intake disturbances are commonly encountered in these patients. The purpose of this study was to determine whether elevation of endogenous NO effects on water intake alterations in cholestatic pationts. 42 mature male rats were randomly divided into 7 groups (N=6)one control group (C), three sham B (B1, B2, B3), and three experimental groups A (A1, A2, A3). After preparation and anesthesia, double ligations were placed on common bile duct in experimental groups, and a loose ligation was done on common bile duct in sham groups. In subgroups A1 and B1, Aminoguanidine (100 mg/kg, IP, for 21 days), in subgroups A2 and B2, N-nitro-L arginine methyl ester (L-Name) (10 mg/kg, IP, for 21 days) and in subgroups A3 and B3 and group C, normal saline 0.9% (the same volume, IP, for 21 days) was administrated. Water intake volume in all groups were detected daily. Data was analysed by Tukey's test and ANOVA. Statistical analysis showed that water intake increased in subgroup A1 and A2 (p