Antidiabetic effect of probioticated persian yellow carrot juice with Lactobacillus acidophilus

Diabetes is a disease characterized by hyperglycemia resulting from defects in insulin secretion or action or both .This resultsin damage dysfunction and failure of various organs especiallyfor the eyes, kidney,heart and blood vessels . Probiotics bacteria defined as live microorganisms which when administered to adequate amounts confer a health benefit on the host. Probiotics have been used for treatment of various type of diarrhea, urogenital infection, gastrointestinal diseas such as, cohn,s disease and pouchitis . Scientist has suggested that LAB containing fermented foods can be involved in the management of chronic metabolic such as a diabetes mellitus disorder.
Yello carrot juice was strilled by 110 , 10 min then inoculums 104 cfu/ml of Lactobacillus acidophilus and incobated in 37 c for 24 h with 5% co2 . Kinetic of Lactobacillus acidophilus growth in carrot juice was measured every 2 hours. Survival of Lactobacillus acidophilus in carrot juice in 4, 25, 37 c was measured every 48 h by cfu / ml . Change of pH, Lactic acid and glucose were measured during storged. The rats keeped at room with 24±2 temperature with 12h light /12h dark cycle. Diabetic situation was confirmed by IP injection of stereptozotocin (STZ). Animals were assigned to a normal group, diabetic group, diabetic group that consumed carrot juice, diabetic group that consumed probioticated carrot juice. After 14 days treatment, the level of the blood glucose was measured.
Probioticated yellow carrot juice could decreased significantly blood glucose level in diabetic rats.Increase in the population of LAB in the intestine as a result of the administration of probioticated carrot juice may result in increased demands for glucose for normal energy requirement of these organism to run their metabolic activities . This may lead to a decrease in the glucose concentration released in to the serum and various organs of the animals. Therefore this research indicates that LAB in carrot juice may be capable for reversing diabetic situation.