Identification of mycobacterium avium subsp paratuberculosis in samples tissue ileum of cattle slaughtered in Shahr-e Kord with ziehl neelsen staining and nested PCR method

Johne s disease is a severe chronic progressive enteritis that infect digestive system of ruminants, domestic animals, wild animals and equine .This study performed due to distinguish M. avium paratuberculosis with nested polymerase chain reaction and Ziehl–Neelsen, s staining on the ilieum samples of slaughtered cattle in Shahr-e- Kord industrial slaughterhouse. Results showed that respectively 6.6% and 26.67 % of bovine are contaminated with M. avium paratuberculosis according to histopathology method and nested polymerase chain reaction . Mac nemar statistical test showed that sensitivity of PCR test was more than histopathological method( P0.05). The results showed that a nested PCR test for diagnosis of infection is high speed and accuracy and in the primary levels of disease that amount of microorganism is a few we cannot trust to result of histopathological method for recognition of Johne‚s disease.