Preparation of human 3D skin matrix and investigation of the interaction between rat adherent bone marrow cells and prepared matrix
Author(s):
Abstract:
Background
Decellularised tissues matrix is considered as an ultimate scaffolding system for two reasons: firstly it has mechanical and structural similarity with natural tissue, secondly extra cellular matrix proteins remain intact after decellularization, which are important for cell differentiation. The aim of this study is investigation behavior rat adherent bone marrow cells cultured in decellularised matrix of human skin.Materials And Methods
In this study, human skin tissues were decellularised by three Methods
physical (slow freezing and snap freezethaw), enzymatic (trypsin 0.25%, 18 hours) and chemical (sodium dodecyl sulfate- SDS 1%, 12 hours). After washing and sterilization procedures, scaffolds cultivated with 4×10⁵ rat adherent bone marrow cells. Finally, histological studies were performed after 1, 2, 3 and 4 weeks of culture and investigated with different staining.Results
Histological study of decellularised scaffolds revealed that nuclear and cellular components of the tissues were completely removed and collagen fibers remained intact. At 1, 2, 3 and 4 weeks of culture, in vitro analysis showed adhesion and also proliferation of rat adherent bone marrow cells. Also, we did not observe any penetration and migration of the cells into scaffolds. Statistical analysis indicated significant difference in percentage of cell density in specimens cultured after 2 weeks versus the other specimens (PConclusion
the overall results showed that remnant ECM (Extracellular Matrix) of human skin can be a suitable scaffold for studying Rat Adherent Bone Marrow Cells behavior.Keywords:
Language:
Persian
Published:
Journal of Sabzevar University of Medical Sciences, Volume:23 Issue: 2, 2016
Pages:
241 to 252
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