Study on the antibacterial effects of silver nanoparticles produced by α-amylase enzyme

With the advent and increasing microbial organisms that resistant to multiple antibiotics, as well as the necessity of decrease the cost of health care, the production of broad range of anti-microbial materials has become unavoidable for human societies. In this study, we synthesized the silver nanoparticles using bacterial α-amylase enzyme and evaluated the antibacterial properties of this eco-friendly nanoparticles.
Silver nanoparticles were synthesized biologically using bacterial α-amylase enzyme, then the effects of antibacterial nanoparticles synthesized in LB medium containing various strains of pathogenic bacteria were investigated and then the minimal inhibitory concentration was calculated.
Nanoparticles produced by this method, based on DLS and SEM analysis, have the size of 20-40 nm and have good activity against Gram-positive bacteria and gram negative pathogenic bacteria. In the 200 of Ag-NPs, the all of pathogenic bacteria was killed.
Biological nanoparticles synthesis relative to other physical and chemical methods has lower cost and higher durability (1). Due to good antibacterial activity of silver nanoparticles, this material has good medical applications.