Evaluation of the Production Methods of Induced Pluripotent Stem Cells: A Short Review

Nowadays, cell therapy is one of the most important and promising strategies in the treatment of diseases. Unique capabilities of stem cells caused them to be used in both research and treatment as a valuable resource in basic science and medical researches.
The use of stem cells has been limited due to the related ethical problems. One of the major concerns of scientists to develop and use more of this science is finding alternative methods for producing these cells. Creating induced pluripotent stem cells (IPSCs) is one of the most important methods in achieving this goal. In this regard, various methods are developing that have the ability to change the gene expression and protein profiles, which results in a change in cells morphology and function in the direction of returning to a undifferentiated status and creating stem cells. These methods include nuclear transfer, use of cell extracts and synthetic molecules, mandatory expression of specific genes, and changes in the levels of cytoplasmic. In this review article, it has been tried to review the methods in the field of the induced pluripotent stem cells production.
Literature search was performed through various combinations of keywords regarding induced pluripotent stem cells, Dedifferentiation methods, and IPSCs generation methods. Papers included in our review cover the period between 2002 to 2015.At the end of the review process, 68 articles were used in our final manuscript. In the present study, we review various methods in the IPS cells generation.