The Comparison of Effect of Chlorhexidine and Proanthocyanidin and Grape Seed Extract on Composite-Dentin Micro Tensile Bond Strength: an in Vitro Experimental Study

Achieving a strong bond between composite resin and dentin remains a challenge in restorative dentistry. The aim of this study was to evaluate the effect of a 2% chlorhexidine (as a matrix metalloproteinase inhibitor), grape seed extract and proanthocyanidin (as a cross-linking agents to further stabilize collagen fibrils) on the dentin bond strength.
Subjects and

The occlusal enamel was removed from 35 human sound premolars and dentin surfaces etched with phosphoric acid and washed with water. Teeth were allocated into seven groups: chlorhexidine, grape seed extract, proanthocyanidin, chlorhexidine plus grape seed extract, chlorhexidine plus proanthocyanidin, ethanol (the solvent of grape seed extract), and left unexposed to any solution (control). Then, a resin composite restoration was made on the dentin surface after using etch-and-rinse adhesive. The micro tensile bond strength (µTBS) of the specimens was analyzed after three month of storage in distilled water and 1500 thermocycles.

Multivariate analysis of variance and t-test showed that control group had a higher bond strength and chlorhexidine plus grape seed extract group had a lowest bond strength than another groups. According to variance analysis chlorhexidine and grape seed extract and proanthocyanidin had comprising effect on dentin bond strength.

Use of chlorhexidin, grape seed extract and proanthocyanidin did not increase the composite-dentine micro tensile bond strength. ►Please cite this paper as: Mapar M, Zakavi F, Farkhondeh Aghideh N, Rahmani Shahriari F, Siahposh A. The Comparison of Effect of Chlorhexidine and Proanthocyanidin and Grape Seed Extract on Composite-Dentin Micro Tensile Bond Strength: an in Vitro Experimental Study.