Optimization of PCR Conditions for CHD-W and CHD-Z for Sex identification in the canary

Bird species are considered sexually monomorphic, so in many bird species, the sex identification is very difficult especially in young birds. Since male canary is more important in warbling, sex identification of canary before puberty is important financially. Molecular genetic techniques can be use for sex identification of animals. Genes on the sex chromosomes are unique because of their sex-specific inheritance. PCR method based on chromo helicase DNA binding protein genes (CHD-Z and CHD-W) has been explained for sex identification in canary. The aim of the present study was to optimize of the PCR conditions for CHD-Z for sex identification in the canary. In this study genomic DNA was extracted from feather bulb of 24 canaries (old and young) of canary. PCR reaction was performed by a set of primers designed on the basis of the sequence of the CHD-W gene and optimized conditions was determined as follow: 94°C, 5min for initial denaturation then 35 cycle of 94°C for 60 sec, 58°C for 35sec, 72°C for 50sec, and a final 72°C for 5min. This study showed optimized PCR can be used for sex identification of canary with high accuracy. Therefore, it is the best method for this purpose.