Investigation of β-Lactoglobulin, Prolactin and DGAT1 genes polymorphism in Khuzestani buffalo

Milk production and its components are belong to quantitative and polygenic traits and affected by many genes. This study was conducted to determine the polymorphism in β-lactoglobulin, prolactin and DGAT1 genes of buffaloes in Khuzestan province using PCR-RFLP technique. Therefore, blood samples were collected from 200 buffaloes of Khuzestan province and then DNA samples were extracted using optimal and modified salting-out method. After DNA extraction, the genotype of the animals was determined by PCR-RFLP. For genotyping of animals, the 262 bp fragment of exon and intron 4 of the beta-lactoglobulin, the 411 bp fragment of exon 8 of the DGAT1 and the 156 bp fragment of exon 3 of the prolactin genes were amplified and then digested using restriction enzyme HeaIII, CfrI and RsaI, respectively. Then restricted PCR products electrophoresed on 2% agaros gel and stained in etediumbromide. Results of this study showed that the allele of B, the allele of A and the allele of K has been fixed in beta-lactoglobulin, prolactin and DGAT1 loci in buffalo population of Khuzestan, respectively and polymorphism was not seen for any of these loci.