Effect of plant growth regulators on plantlet regeneration from callus in two Iranian melons cv. (Cucumis melo) by In-vitro

Melon is one of the important species of cucurbitaceae that have been used for transgenic and studies of regeneration by in-vitro. For callus production in two Iranian melon cultivars, explants of cotyledons prepared from Eivanaky and Zard-e-jalali and had been cultured in callus induction medium, with basal MS medium that supplemented with different combination of IAA and BA. Morphogen calli production from cotyledonary explants has been used for regeneration after one month. About one gram morphogen callus transferred to regeneration media that includes, MS medium supplemented with combination of three different concentrations of IAA (0, 0.05 and 0.5 mg/l) and three different concentration of BA (0.1, 0.5 and 1 mg/l). After two months regeneration percentage was evaluated. Results showed that cultivars, IAA and BA concentrations and interaction between them had significant effects on regeneration. Also, results showed independently of cultivars and concentrations of plant growth regulators, the Eivanaki cultivar had highest regeneration with average of 40% in compared with Zard-e-Jalali with average of 31%. However, combination of 0 mg/l IAA 1 mg/l BA with means of 70% and then 0.05mg/l IAA 0.5 mg/l BA with average of 62% had highest regeneration of shoots in both cultivars.