The effect of six plant growth regulators on somatic embryogenesis in Eucalyptus rubida

Since Iran in comparison with other countries is a country with low cover forest, using fast-growing species of Eucalyptus could play a key role to extend such coverage. However, micropropagation methods applied for colon production of best-qualified verity and/or species of Eucalyptus by many researchers, because of difficulties in Eucalyptus propagation through usual methods. The possibility of micro propagation of Eucalyptus rubida (Candlebark Gum) through somatic embryogenesis was investigated for the first time. Leaf cotyledon, stem and root explants of un-contaminated E. rubida plantlets were cultured on semi-solid MS media with 36 hormone treatments and 3 MS liquid media, all together 39 MS media. The best results obtained in liquid MS media supplemented with 0.2 mg/l BA, 1 mg/l 2,4-D and ABA by root explant's calli on the first medium with 2,4-D and TDZ. Embryogenic calli proceeded three global, heart-shaped and torpedo stages.