Study on the intestinal yeast flora of domestic rainbow trout from Urmia city with emphasizing to identification of Rhodotorula, Saccharomyces and Candida genuses

Unicellular yeasts are known as a part of aquatics intestinal flora. Some species are useful and some are known as pathogen for known or unknown diseases. So far any comprehensive study was done to characterize yeast intestine flora of fish in Iran. Due to the importance of isolation and characterization of micro flora of fishes and possibility of introducing this endemic species as Probiotics, in present study, the diversity of the intestinal yeast flora of domestic Rainbow trout fishes of 6 Urmia cities farms was investigated by general and specific diffraction media as SDA, SCC, CHA and CMA following to molecular analyses. Molecular technique of PCR for small fragment of ITS1 was followed by sequencing in order to identifying some complex samples in case of culture methods failure. This study not only succeed to stablishe a protocol for characterization of yeast micro flora of fishes but also could identify some species Candida guilliermondii, Saccharomyces cerevisiae strain LN, Saccharomyces cerevisiae strain YG3-1, Saccharomyces cerevisiae and also strains of Rhodotorula.