Extraction and determination of chlorogenic acid in Echinacea purpurea using a novel solid phase micro extraction method

The study has conducted based on the development and application of a novel extraction technique to determine the phenolic compounds in Echinacea purpurea herbal extract. A composite adsorbent based on solid-phase micro-extraction techniques using sol-gel was prepared. The composite was homogeneously injected into a polypropylene hollow fiber. Finally, the device was used as a selective sorbent to extract the chlorogenic acid as a phenolic compound in E. purpurea. Thereafter, the chlorogenic acid amounts in the extract was measured by high performance liquid chromatography (HPLC). Main factors affecting the synthesis of the composite and factors affecting the microextraction process were studied and optimized. The detection limit of the technique was 0.08 ng. mL-1. Linear range and relative standard deviation were 0.2 -1000 ng. ml-1 and 0.38% (n=3), respectively. The average relative recovery of the analyte added to the sample at four concentration levels were between 84.4 to 97.2 percent. This method was proposed as a new method to determine chlorogenic acid and other phenolic compounds in E. purpurea and other plants.