Detection of Infectious Bronchitis Virus B/793 Serotype in Broiler Chicken in Shiraz by Molecular Methods

Avian infectious bronchitis (IB) is an acute and contagious disease, and can be involved respiratory and urogenital systems. It can be cause high mortality with economic losses in industrial poultry. For IB detection, tracheal and lung tissue samples were collected from 30 broiler chicken farms with respiratory syndrome in Shiraz and transported to laboratory in cold chain. After preparation and inoculation of samples into embryonated SPF eggs, samples checked for avian influenza (AI) and Newcastle disease (ND) by hemagglutination (HA) test. Viral RNA was extracted from allantoic fluid by purification kit and RT-PCR optimized for detection infectious bronchitis virus (IBV) with global primers and used specific primers for detection of massachusetts and B/793 serotypes by nested-PCR. From 30 broiler chicken flocks, 6 flocks were positive for IBV, and 4 flocks were positive for B/793 serotype in nested-PCR. Association to this result, use of vaccine with B/793 serotype is necessary for chickens.