The effect of some chemical factors on serinoproteinases activity of trypsin and chymotrypsin in Caspian brown trout (Salmo trutta caspius) fry

The effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors was considered on trypsin and chymotrypsin activity of the Caspian brown trout fry. The results showed K and Na didn’t significanly decrease trypsin and chymotrypsin activity (p>0.05). Ca2 and Mg2 significantly increased trypsin and chymotrypsin activity (p0.05). Oxidizing agents, including hydrogen peroxide and sodium perborate, significantly decreased trypsin (p0.05). The inhibitors such as TPCK, pepstatinA, iodoacetic acid, EDTA and ß-mercaptoethanol did not significantly decrease the trypsin activity (p>0.05), but they significantly decreased chymotrypsin activity (p0.05).