Cytological Developments Of Microspore In Four Varieties Of Tomato (Lycopersicum esculentum Mill)

Homozygous doubled haploid lines production through induction of androgenesis is a promising method to accelerate the classical breeding programs. However, this technology is relatively under-developed in tomato so that improvements in methodology are required. One of the critical factors for induction of androgenesis in tomato is to use of microspores being in appropriate developmental stage. Cytological examination is one of the most accurate methods for determining the correct stage of microspore development. In this study, a number of characteristics were evaluated including the cytological properties of normal microspores development and pollen grains and the relationship between length of flower bud and anther length. Flower buds with 2.0-7.9 mm in size were divided into six groups by one mm intervals. In each group three flower buds randomly selected and length of anthers were measured. Flower buds were incubated at 4oC for 15 minutes and stained in %4 acetocarmine solution. The results showed that there is a significant correlation between length of flower bud and anther length (P