The Effect of Fe2O4Zn Nanoparticles on Kidney Tissue and Blood Biochemical Factors in Wistar Male Rats

Nanoparticles should be investigated under laboratory conditions for using in treatment of diseases. The aime of the study was to investigate the effects of nanoparticles of iron-zinc (Fe2O4Zn) on the factors kidney male rats Wistar. In this experimental study, 24 male Wistar rats were randomly divided into 3 groups which contain two experimental groups and one control group. The control group received 0.5 ml of saline solution but others groups recieved 0.5 ml of iron nanoparticle with concentration of 100ppm and 200ppm, respectively for 7 consecutive days that were injected intraperitoneally. On the second, seventh and fourteenth day after treatment, blood from the corner of the eyelids was done with of capillary tube. Urea, uric acid and creatinine were measured by ELISA method. Results of statistical analysis showed that urea and uric acid levels in the seventh and fourteenth days after treatment at a dose of 100ppm and 200ppm were decreased. The creatinine values ​​at days 2, 7 and 14 days after treatment 100ppm and 200ppm were increased. At a Low dose, nanoparticles sediment in the connective tissue and cytoplasm of proximal and distal renal cell and at high doses of nanoparticles, deposit is made in cells of the proximal tubule and in the central part of kidny. Levels of urea and uric acid were reduced and creatinine was increased. The results of histological showed nano-particle deposition in the kidney. It seems that the application of Fe2O4Zn nanoparticles in the biological system has no stable and long-term toxic effects on the animal body.