The Teratogenic Effects Pesticide of Chlorpyrifos on the Development of Balb/C Mouse Embryos 3Th to 6Th Days of Pregnancy

Chlorpyrifos is one of organophosphate Insecticides and had studied in recent years because it has many complications devastating on skin, nervous, respiratory and digestive systems, but it effects on the embryo especially during pregnancy has not been reviewed. Therefore in study the teratogenic effects pesticide of Chlorpyrifos on the development of Balb/C mouse embryos 3th to 6thdays of pregnancy. In this study 50 female Balb/C mouse were randomly divided into 6 equal groups a control group (non-injection: 5 mice) and witness (injection of saline: 5 mice) and 4 experimental groups (each group: 10 mice). A lethal dose LD50 was determined in condition of 25.32 ml/kg.bw in vivo and selected dose for injection 0.4 ml/kg.bw. Injection was done on the 3Th to 6Th days of pregnancy days by enema. Then the mouse were sacrificed on day 15 of pregnancy.For security of results above experiences was repeated 2times. Data was checked with SPSS17 software by percentage. After a comparative study at this dose, has been observed a significant increase, exohepatic, exencephalus, Syndactyly, having a defect in dynamic organs (legs and hands), extensive bleeding in whole body and Exophthalmia were compared with control and witness groups.According to the findings, negative effects of Chlorpyrifos use as pesticides in agricultural products, On the mouse embryos and It is recommended to protect the environment and human health,especially pregnant women use of non-chemical methods to control pests.