Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during 2013-2015

Streptococcus pneumoniae is Gram-positive bacterium that causes pneumonia, septicemia, meningitis, otitis media, sinusitis, bronchitis, bacteremia and infectious processes is introduced. Due to the clinical importance of this bacterium and correct and timely diagnosis and separation of isolated samples from patients, this study were performed to identify and isolate strains of Pneumococcus from clinical samples by culture and PCR methods.
In this study, 175 various clinical samples were collected from admitted patients in Tehran hospitals during 3 years (2013-2015),and culture and PCR methods used to isolate and identify of strains of Pneumococcus (after genome extraction and in the presence of specific primer) beside the standard strain ATCC 49619 (as control).
In this study, 40 strains of Pneumococcus have been identified and approved by culture and PCR methods. Amplified amplicon in this study, was a 160 bp fragment that were found in all strains of Streptococcus pneumoniae. Also, the PCR product was confirmed by sequencing.
According to the obtained results in this study, PCR similar to culture, is a reliable method, but has been assessed relatively faster that can be used in the future to replace the old and conventional methods (biochemical tests and culture).