Study of Antibacterial Effect of Aqueous, Ethanolic and Hydroalcoholic Extracts of Aerial Organs of Salvia chorassanica against Some Spoilage and Poisoning Bacteria

The purpose of this study is to investigate the antimicrobial effect of the aqueous, ethanolic and hydroalcoholic extracts of aerial organs of Salvia chorassanica against Staphylococcus aureus (ATCC: 6538), Enterococcus faecalis (ATCC: 21299), Salmonella typhimurium (ATCC: 14028) and Escherichia coli (ATCC: 25922). In this study the antibacterial effect of the extracts was determined using the agar diffusion method. Microdilution method by ELISA and adding phenyl Tetrazolium chloride reagent was used to determine the minimum inhibitory concentration and minimum bactericidal concentration. The highest inhibition zone in diffusion method was related to aqueous and hydroalcoholic extracts of aerial parts of Salvia chorassanica against Enterococcus faecalis. The MIC in aqueous and ethanolic extracts of the aerial parts for Gram-Positive was 60 mg/ml, for Escherichia coli 60 and 120 mg/ml and for Salmonella typhimurium 120 and 240 mg/ml, respectively. The amount in hydroalcoholic extracts for Gram-Positive bacteria was 60 mg/ml and for Gram-Negative bacteria was 120 mg/ml.