The study of structural characteristics and Allergenicity potential of Quercus brantii L. pollen grains

Allergy is among those diseases caused by different substances such as pollen affecting many people worldwide where they are living next to such pollen-distributing plants. Quercus brantii is one of the most common trees growing in different parts of Iran including Ilam. The current study aimed to evaluate the variations of IgE and some cytokines such as IFN-Y and TNF-a in rats exposed to the pollen of this tree. Pollens were collected from different parts of Ilam. The inflorescence, in different developmental stages, were removed, fixed inFAA, stored in 70% ethanol, embedded in paraffin and then sliced by microtome. Staining was carried out with PAS and contrasted with Hematoxylin. Rats included in five groups, optical, dermal, nasal, serological and control group with 5 rats in each group. Pollen essence was extracted and applied for each group and 2hrs, 24hrs and one week after exposure, serum samples were collected while the reactions of each rat for symptoms of allergy were recorded too. ELISA was employed to measure nephlomety analysis was applied for total IgE. Data were analyzed by SPSS using ANOVA, Mean evaluation and Pearson correlation. Microscopic examination demonostrated three colpus pollens of plant caet with 94.30 µm in diameter and there are granoralexine in pollens surface. Wheal and flares was observed by 75mg-L of the pollen subcutaneously eye and nasal allergy symptoms were seen in optical and nasal groups, together with sneezing. Total IgE for the serology group was 16.50. IFN and TNF were also different in case and control groups indicating the variation of these immunological properties due to the pollen of this tree. The pollen of Quercus brantii is allergeric when it is inhaled or is in contact with skin and also even when it is injected.