Evaluation of Effective Factors on Survival and Activity of Petroleum Degradation Bacteria in Bioremediation Process

Tang Fany petroleum Pumping Station (TFOPS) of Poldokhtar is an important and the old point of oil transition. The soil and water around this site is petroleum polluted due leakage of petroleum. The purpose of this study was to evaluate effective factors for survival and fitness of the petroleum degrading bacteria. The following bacterial species were isolated and identified from petroleum polluted soil and water in TFOPS region: Pseudomoas aeruginosa, Acinetobacter junii, Acinetobacter buumanii, Delftia tsuruhatensis, Comamonas Koreensis, Sphinobacterium multivorum and Stenotrophomonas acidaminiphila. The MIC and MBC were determined relative to oil whit the macrodilution method. The bacterial species Compared and evaluated on the factors ability to form a bacterial biofilm, siderophore production, indole acetic acid and dissolve phosphate. Two species namely P. aeruginosa and C. koreensis showed the highest and the least petroleum concentration tolerance, respectively. The two species also have the highest and the least biofilm production ability, respectively. Acinetobacter junii and A. baumannii in the test of dissolution of phosphate, most were able to dissolve phosphate. Sphingobacterium multivorum highest produced indole acetic acid. Due to the ability of the bacterial species to tolerate oil pollution and factors to promote plant growth, it can be suggested as alternative for application in the Bioremediation of contaminated soil events.
a low differentiation between two populations of this species in the studied areas due to high level of gene flow (Nm=18.205) between two regions and low Fst (0.036). Also, analysis of molecular variance showed there is low genetic variation among populations and most of the observed variation is within the populations.